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KMID : 1145520150010020104
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Journal of Radiopharmaceuticals and Molecular Probes
2015 Volume.1 No. 2 p.104 ~ p.108
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Development of 68Ga-human serum albumin as a PET imaging agent for diagnosis of acute inflammation
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Lee Ji-Youn
Kim Ho-Young
Lee Bo-Eun
Kim Young-Ju
Lee Yun-Sang
Jeong Jae-Min
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Abstract
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Human serum albumin (HSA) has potential for diagnosis and therapy in clinical setting. The purpose of experiments was to develop and evaluate 68Ga-HSA as a PET agent for diagnosis of acute inflammation. NOTA-HSA was synthesized by conjugating 2-(p-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid to HSA in 0.1 M sodium carbonate buffer (pH 9.5) and then purified using a PD-10 size-exclusion column. NOTA-HSA was labeled with 68Ga at room temperature for 10 min, and 8.4% sodium hydrogen carbonate buffer was added for neutralization. 68Ga-NOTA-HSA was purified using alumina N plus light cartridge and 0.22 μm syringe filter. Labeling efficiency and radiochemical purity were determined by ITLC-SG with 0.1 M citric acid. Biodistribution study was performed in a male BALB/c mice model of Carrageenan-induced acute inflammation. Animal PET study was performed in acute inflammation mice model after tail vein injection of 68Ga-HSA. This radiotracer showed high labeling efficiency (>99%) around pH 7. Biodistribution study showed higher inflamed footpad uptake than control footpad uptake. Animal PET study revealed 2 times higher uptake on inflamed footpad compared to control footpad. In these experiments, we developed 68Ga-HSA for acute inflammation PET imaging and evaluated it in a mouse disease model. The results demonstrated that 68Ga-HSA has potential as a PET imaging agent for diagnosis of acute inflammation.
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KEYWORD
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68Ga, Human serum albumin (HSA), Positron emission tomography, Inflammation
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